LOCUS       Exported                4491 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-909, complete sequence.
ACCESSION   AY741665
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Shigaki T, Vyzasatya RR, Sivitz AB, Ward JM, Sze H, Hirschi KD.
  TITLE     The Cre-loxP recombination-based reporter system for plant 
            transcriptional expression studies
  JOURNAL   Plant Mol. Biol. 58 (1), 65-73 (2005)
  PUBMED    16028117
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Shigaki T, Hirschi KD, Ward JM, Sze H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2004) Pediatrics, Baylor College of Medicine, Room
            9016, CNRC, 1100 Bates St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 4491)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Cloning vector p713-909"
                     /mol_type="other DNA"
                     /note="used to clone a promoter fragment for Cre-loxP 
                     recombination"
                     /db_xref="taxon:295514"
     misc_feature    1..3424
                     /label=derived from cloning vector pUNI(Amp)-GFP
                     /note="derived from cloning vector pUNI(Amp)-GFP"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             67..810
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /note="GFP"
                     /protein_id="AAV27315.1"
                     /translation="MAMGIPGLINSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGD
                     ATYGKLTLKFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA
                     DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE
                     KRDHMVLLEFVTAAGITHGMDELYK"
     CDS             97..810
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     polyA_signal    925..1132
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      1204..1251
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1347..1703
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1756..2211)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2245..3105)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance marker"
                     /protein_id="AAV27316.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPFRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3106..3210)
                     /gene="bla"
                     /label=AmpR promoter