LOCUS       Exported                3341 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-1555, complete sequence.
ACCESSION   DQ062657
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Shigaki T, Kole M, Hirschi KD.
  TITLE     Cre-loxP recombination vectors for the expression of Riken 
            full-length plant cDNAs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Shigaki T, Kole M, Hirschi KD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2005) Childrens Nutrition Research Center, Baylor
            College of Medicine, Room 9016, 1100 Bates St., Houston, TX 77030, 
            USA
REFERENCE   3  (bases 1 to 3341)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Cloning vector p713-1555"
                     /mol_type="other DNA"
                     /db_xref="taxon:331655"
     protein_bind    127..160
                     /label=LoxP binding site
                     /bound_moiety="LoxP"
     misc_feature    183..903
                     /note="stuffer DNA from Arabidopsis thaliana cation/proton 
                     exchanger CAX2"
     regulatory      922..1176
                     /regulatory_class="terminator"
                     /note="nos terminator"
     terminator      922..1174
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      1247..1294
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1379..1785
                     /label=R6K-gamma
                     /note="R6K-gamma"
     rep_origin      1390..1746
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1793..2248)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2282..3142)
                     /codon_start=1
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=beta-lactamase
                     /protein_id="AAY66997.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3143..3247)
                     /gene="bla"
                     /label=AmpR promoter