LOCUS       Exported                3575 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Suicide vector p6Sui2, complete sequence.
ACCESSION   KX863719
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Fleige C, Meyer F, Steinbuchel A.
  TITLE     Metabolic Engineering of the Actinomycete Amycolatopsis sp. Strain 
            ATCC 39116 towards Enhanced Production of Natural Vanillin
  JOURNAL   Appl. Environ. Microbiol. 82 (11), 3410-3419 (2016)
  PUBMED    27037121
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Fleige C, Meyer F, Steinbuechel A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2016) Institut fuer molekulare Mikrobiologie und 
            Biotechnologie, Westfaelische Wilhelms-Universitaet Muenster, 
            Corrensstrasse 3, Muenster, NRW 48149, Germany
REFERENCE   3  (bases 1 to 3575)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Suicide vector p6Sui2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1925252"
     CDS             293..580
                     /codon_start=1
                     /gene="int3"
                     /product="putative Int3-like integrase"
                     /label=int3
                     /protein_id="APH11644.1"
                     /translation="MPINITIFVETIGAAIYPQDISTPSYIEAESTRFFALRELHQVGD
                     AVELFDQKLLDRRRGEFRLFHISLPPDERLLRHSPSAVPIGSAVQMRDFR"
     gene            293..580
                     /gene="int3"
                     /label=int3
     CDS             complement(719..1522)
                     /codon_start=1
                     /gene="aacC4"
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /label=aacC4
                     /note="confers resistance to apramycin and kanamycin"
                     /protein_id="APH11643.1"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     gene            complement(719..1522)
                     /gene="aacC4"
                     /label=aacC4
     CDS             complement(722..1522)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1789..2765
                     /label=pBBR origin of replication for E. coli
                     /note="pBBR origin of replication for E. coli"
     rep_origin      complement(2077..2665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2766..2943
                     /note="partial sequence of the pA387 origin of replication 
                     for Amycolatopsis; nonfunctional"
     misc_feature    3174..3289
                     /gene="aacC1"
                     /label=similar to aminoglycoside-(3)-acetyltransferase
                     /note="similar to aminoglycoside-(3)-acetyltransferase"
     gene            3174..3289
                     /gene="aacC1"
                     /label=aacC1