LOCUS       Exported                8108 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p5E-twhh, complete sequence.
ACCESSION   JX261972
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Sztal TE, Sonntag C, Hall TE, Currie PD.
  TITLE     Epistatic dissection of laminin-receptor interactions in dystrophic 
            zebrafish muscle
  JOURNAL   Hum. Mol. Genet. 21 (21), 4718-4731 (2012)
  PUBMED    22859503
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Hall TE, Sztal TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2012) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Cloning vector p5E-twhh"
                     /mol_type="other DNA"
                     /db_xref="taxon:1219763"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     536..553
                     /label=M13-fwd
                     /note="M13-fwd"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     668..688
                     /label=attB4
                     /note="attB4"
     regulatory      689..6153
                     /regulatory_class="promoter"
                     /note="zebrafish tiggy-winkle hedgehog (twhh) enhancer"
     protein_bind    6153..6277
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     6154..6174
                     /label=attB1
                     /note="attB1"
     primer_bind     complement(6349..6370)
                     /label=T7
                     /note="T7"
     promoter        complement(6350..6368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6369..6389)
                     /label=M13-rev
                     /note="M13-rev"
     primer_bind     complement(6373..6389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6502..7311
                     /codon_start=1
                     /product="aminoglycoside 3-phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aminoglycoside 3-phosphotransferase
                     /note="Kan R+"
                     /protein_id="AFP72867.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7432..8105
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      7458..8046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"