LOCUS       Exported                6618 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p5E-acta1, complete sequence.
ACCESSION   JN689239
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Jacoby AS, Busch-Nentwich E, Bryson-Richardson RJ, Hall TE, Berger 
            J, Berger S, Sonntag C, Sachs C, Geisler R, Stemple DL, Currie PD.
  TITLE     The zebrafish dystrophic mutant softy maintains muscle fibre 
            viability despite basement membrane rupture and muscle detachment
  JOURNAL   Development 136 (19), 3367-3376 (2009)
  PUBMED    19736328
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Hall TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2011) Australian Regenerative Medicine Institute, 
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   3  (bases 1 to 6618)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Cloning vector p5E-acta1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1096444"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="M13 Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     593..688
                     /label=attL4
                     /note="attL4"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      689..4663
                     /regulatory_class="promoter"
                     /note="zebrafish skeletal muscle alpha actin promoter"
     misc_recomb     4464..4684
                     /label=attB1
                     /note="attB1"
     protein_bind    4663..4787
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     4664..4820
                     /label=attR1
                     /note="attR1"
     primer_bind     4859..4880
                     /label=T7
                     /note="T7"
     promoter        complement(4860..4878)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4879..4899
                     /label=M13 Reverse
                     /note="M13 Reverse"
     primer_bind     complement(4883..4899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5012..5821
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /protein_id="AER25339.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      5942..6615
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      5968..6556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"