LOCUS       Exported                5909 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p53-luc, complete sequence.
ACCESSION   AF081837
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Zheng C-F., Grafsky A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-1998) Technical Services, Stratagene, 11011 N. 
            Torrey PInes Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Zheng C-F., Grafsky A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-DEC-1998) Technical Services, Stratagene, 11011 N. 
            Torrey PInes Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 5909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Dec 30, 1998 this sequence version replaced gi:3548971.
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Cloning vector p53-luc"
                     /lab_host="Escherichia coli K12"
                     /mol_type="genomic DNA"
                     /note="synthetic plasmid maintained and amplified in 
                     Escherichia coli; cis-acting vector in Stratagenes 
                     PathDetect system"
                     /db_xref="taxon:80749"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             137..997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1934..2074
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2541..2673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3030..4682
                     /codon_start=1
                     /product="luciferase"
                     /label=luciferase
                     /protein_id="AAC98686.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4813..4878
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5448..5582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     RBS             5617..5625
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"