LOCUS       Exported                6412 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast expression vector p426GALL, complete sequence.
ACCESSION   DQ023608
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Mumberg D, Muller R, Funk M.
  TITLE     Yeast vectors for the controlled expression of heterologous proteins
            in different genetic backgrounds
  JOURNAL   Gene 156 (1), 119-122 (1995)
  PUBMED    7737504
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Ralser M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2005) Dep. Lehrach, Max- Planck Institute for 
            Molecular Genetics, Ihnestrasse 73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 6412)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Yeast expression vector p426GALL"
                     /mol_type="other DNA"
                     /db_xref="taxon:329402"
     promoter        201..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             417..1220
                     /codon_start=1
                     /product="URA3"
                     /label=URA3
                     /note="orotidine-5-phosphatase"
                     /protein_id="AAY44380.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1807)
                     /direction=LEFT
                     /label=F1_ORI
                     /note="F1_ORI"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     1951..1967
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1977..1996
                     /regulatory_class="promoter"
                     /note="T7_P"
     promoter        1977..1995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(2010..2261)
                     /regulatory_class="terminator"
                     /note="CYC1-terminator"
     terminator      2014..2261
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     2264..2280
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2314..2330)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(2331..2791)
                     /regulatory_class="promoter"
                     /note="GALL"
     promoter        complement(2345..2786)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2669..2786
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     regulatory      complement(2809..2828)
                     /regulatory_class="promoter"
                     /note="T3_P"
     promoter        complement(2810..2828)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2849..2865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(2854..2975)
                     /regulatory_class="promoter"
                     /note="lac"
     protein_bind    2873..2889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2897..2927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      3069..4009
                     /label=PMB1
                     /note="PMB1"
     rep_origin      complement(3251..3839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4010..4870)
                     /codon_start=1
                     /product="AP(R)"
                     /label=AP
                     /note="bla beta-lactamase; ampR ampicillin resistance"
                     /protein_id="AAY44381.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4871..4975)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5002..6347
                     /label=2_MICRON
                     /note="2_MICRON"
     rep_origin      5002..6344
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5965..6012)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."