LOCUS       Exported                3253 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p3E-zfRab18a-R93del, complete sequence.
ACCESSION   KM014496
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Nixon SJ, Hall TE, Parton RG.
  TITLE     Rab18 in zebrafish
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Hall TE, Parton RG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2014) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 3253)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Cloning vector p3E-zfRab18a-R93del"
                     /mol_type="other DNA"
                     /db_xref="taxon:1545816"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     536..553
                     /label=M13-fwd
                     /note="M13-fwd"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     complement(727..747)
                     /label=AttB2
                     /note="AttB2"
     CDS             747..1361
                     /codon_start=1
                     /product="Rab18a"
                     /label=Rab18a
                     /note="with R93del mutation; derived from zebrafish"
                     /protein_id="AIO08314.1"
                     /translation="MNDDILTTLKILIIGESGVGKSSLLLRFTDDTFDAEIGATIGVDF
                     KVKTLAVDGNRAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVSRETFAKLDNWLSE
                     LETYCTRNDLVKMLVGNKIDKDDREIEREEGLKFARKHSMLFIESSAKTRDGVQCAFEE
                     LVEKILQTPGLWESMHKSRGVALSELSESSQGGCGAYCSLL"
     misc_recomb     complement(1362..1457)
                     /label=AttL3
                     /note="AttL3"
     protein_bind    complement(1362..1457)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     complement(1362..1382)
                     /label=AttB3
                     /note="AttB3"
     primer_bind     complement(1494..1515)
                     /label=T7
                     /note="T7"
     promoter        complement(1495..1513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1514..1534)
                     /label=M13-rev
                     /note="M13-rev"
     primer_bind     complement(1518..1534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1647..2456
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /note="Kan R+"
                     /protein_id="AIO08313.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2577..3250
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      2603..3191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"