LOCUS       Exported                5167 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector p35S-loxP-Zeo DNA, complete sequence.
ACCESSION   AB540628
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Moss transformation vector p35S-loxP-Zeo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2010) Contact:Yuji Hiwatashi National Institute 
            for Basic Biology, Evolutionary Biology; Myodaiji, Nishigonaka, 38, 
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 5167)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Moss transformation vector p35S-loxP-Zeo"
                     /mol_type="other DNA"
                     /db_xref="taxon:706411"
     misc_feature    1..660
                     /label=pBluescript SKII
                     /note="pBluescript SKII"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    708..741
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     708..714
                     /label=loxP
                     /note="loxP"
     primer_bind     816..832
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(834..1579)
                     /regulatory_class="terminator"
                     /note="cauliflower mosaic virus 35S polyA signal"
     polyA_signal    1344..1520
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1590..1964)
                     /codon_start=1
                     /gene="ble"
                     /product="zeocin resistance protein"
                     /label=ble
                     /protein_id="BAI66366.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVLVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            complement(1590..1964)
                     /gene="ble"
                     /label=ble
     regulatory      complement(1971..2786)
                     /regulatory_class="promoter"
                     /note="cauliflower mosaic virus 35S promoter"
     promoter        complement(1971..2016)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_recomb     2854..2887
                     /label=loxP
                     /note="loxP"
     protein_bind    2854..2887
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2938..5167
                     /label=pBluescript SKII
                     /note="pBluescript SKII"
     promoter        complement(2979..2997)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3018..3034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3042..3058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3066..3096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3111..3132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3420..4008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4179..5039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5040..5144)
                     /gene="bla"
                     /label=AmpR promoter