LOCUS       Exported                4518 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p35S-GFP, complete sequence.
ACCESSION   U28417
VERSION     .
KEYWORDS    Green fluorescent protein; GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Niedz RP, Sussman MR, Satterlee JS.
  TITLE     Green fluorescent protein: an in vivo reporter of plant gene 
            expression
  JOURNAL   Plant Cell Rep. 14 (7), 403-406 (1995)
  PUBMED    24185445
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disk version 1.3
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4518)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-1995) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            4030 Fabian Way, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 4518)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 4030 
            Fabian Way, Palo Alto, CA 94303, USA. To place an order call (415) 
            424-8222 or (800) 662-2566, extension 1. International customers, 
            please contact your local distributor. For technical information, 
            call (415) 424-8222 or (800) 662-2566, extension 3. This sequence 
            has been compiled from information in the sequence databases, 
            published literature and other sources, together with partial 
            sequences obtained by CLONTECH. If you suspect there is an error in 
            this sequence, please contact CLONTECHs Technical Service 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Cloning vector p35S-GFP"
                     /plasmid="p35S-GFP"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:41037"
     promoter        512..857
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             896..1612
                     /codon_start=1
                     /gene="gfp"
                     /product="green fluorescent protein"
                     /label=gfp
                     /protein_id="AAA69675.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            896..1612
                     /gene="gfp"
                     /label=gfp
     terminator      1629..1881
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1890..1906)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2380..2484
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2485..3345
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicillin"
                     /label=bla
                     /protein_id="AAA69676.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2485..3345
                     /gene="bla"
                     /label=bla
     rep_origin      3516..4104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4392..4413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4428..4458
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4466..4482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4490..4506
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"