LOCUS       Exported                4635 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector p35S-BSD2 DNA, complete sequence.
ACCESSION   LC279014
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Koshimizu S, Aono N, Sasaki-Sekimoto Y, Shigenobu S, Shimojima M, 
            Ohta H, Kofuji R, Tamada Y, Murata T, Hasebe M.
  TITLE     Physcomitrella MADS-box genes regulate external water conduction and
            sperm movement necessary for fertilization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Hiwatashi Y, Koshimizu S, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) Contact:Shizuka Koshimizu National Institute
            for Basic Biology, Division of Evolutionary Biology; Nishigonaka 38,
            Myodaiji, Okazaki, Aichi 444-8585, Japan URL 
            :http://www.nibb.ac.jp/evodevo/
REFERENCE   3  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Moss transformation vector p35S-BSD2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2014870"
     misc_feature    1..712
                     /label=pBluescript SKII+
                     /note="pBluescript SKII+"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      718..1046
                     /regulatory_class="promoter"
                     /note="cauliflower mosaic virus 35S promoter"
     promoter        766..1111
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1147..1539
                     /codon_start=1
                     /gene="BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /protein_id="BBC14925.1"
                     /translation="MPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVN
                     VYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKA
                     IVKDSDGQPTAVGIRELLPSGYVWEG"
     gene            1147..1539
                     /gene="BSD"
                     /label=BSD
     regulatory      1540..2392
                     /regulatory_class="terminator"
                     /note="cauliflower mosaic virus 35S polyA signal"
     polyA_signal    1682..1858
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2393..4635
                     /label=pBluescript SKII+
                     /note="pBluescript SKII+"
     primer_bind     complement(2394..2410)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2447..2465)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2486..2502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2510..2526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2534..2564)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2579..2600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2888..3476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3647..4507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4508..4612)
                     /gene="bla"
                     /label=AmpR promoter