LOCUS       Exported                3808 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p34E-TpTer, complete sequence.
ACCESSION   KX485329
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira 
            T, Bull EH, Butt AT, Thomas MS.
  TITLE     An efficient system for the generation of marked genetic mutants in 
            members of the genus Burkholderia
  JOURNAL   Plasmid (2016) In press
  PUBMED    27825973
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Shastri S, Spiewak HL, Pereira T, Sofoluwe AO, Eidsvaag VA, Bull EH,
            Asghar AH, Thomas MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2016) Department of Infection, Immunity & 
            Cardiovascular Disease, The University of Sheffield, The Medical 
            School, Beech Hill Road, Sheffield, South Yorkshire S10 2RX, UK
REFERENCE   3  (bases 1 to 3808)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Cloning vector p34E-TpTer"
                     /mol_type="other DNA"
                     /db_xref="taxon:1918675"
     regulatory      786..890
                     /regulatory_class="promoter"
                     /note="bla promoter"
     promoter        786..890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             891..1751
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers resistance to ampicillin"
                     /protein_id="APD15315.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            891..1751
                     /gene="bla"
                     /label=bla
     rep_origin      1922..2510
                     /note="oriR; ColE1/pMB1/pBR322/pUC origin of replication"
     regulatory      2762..2780
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        2762..2780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2789..2845
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      2890..2917
                     /regulatory_class="terminator"
                     /note="rrnB T2 terminator; transcription terminator T2 from
                     the E. coli rrnB gene"
     terminator      2890..2917
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3009..3095
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      3049..3092
                     /regulatory_class="terminator"
                     /note="rrnB T1 terminator; transcription terminator T1 from
                     the E. coli rrnB gene"
     CDS             complement(3119..3355)
                     /codon_start=1
                     /gene="dfrB2"
                     /product="dihydrofolate reductase"
                     /label=dfrB2
                     /note="confers resistance to trimethoprim"
                     /protein_id="APD15316.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     gene            complement(3119..3355)
                     /gene="dfrB2"
                     /label=dfrB2
     regulatory      complement(3681..3709)
                     /regulatory_class="promoter"
                     /note="PcS promoter"
     promoter        complement(3681..3709)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    3729..3785
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      complement(3790..3808)
                     /regulatory_class="promoter"
                     /note="SP6 promoter"
     promoter        complement(3790..3808)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"