LOCUS       Exported                5686 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector p2619-EO6, complete sequence.
ACCESSION   DQ115382
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5686)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Shuttle vector p2619-EO6"
                     /mol_type="other DNA"
                     /note="Mycobacterium-E. coli shuttle vector; Murine EO 
                     rotavirus VP6 cloned into BamH1-Hind111 sites of p2619#"
                     /db_xref="taxon:336700"
     misc_feature    7..1219
                     /note="APH1 region of Tn903 encoding 
                     aminoglycoside-3-phosphotransferase, conferring kanamycin 
                     resistance"
     CDS             121..936
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1224..2010
                     /label=OriE region derived from pUC19
                     /note="OriE region derived from pUC19"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2029..3927
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3960..4200
                     /regulatory_class="promoter"
                     /note="derived from Mycobacterium bovis hsp60 promoter of 
                     GenBank Accession Number M17705"
     misc_feature    4200..4344
                     /note="similar to Mycobacterium tuberculosis 19 kDa 
                     antigen"
     CDS             4262..5548
                     /codon_start=1
                     /product="19kDa antigen/VP6 chimera"
                     /label=19kDa antigen/VP6 chimera
                     /note="21 amino acid signal sequence plus 7 amino acids of 
                     mature Mycobacterium tuberculosis 19 kDa antigen linked via
                     3 additional amino acids to 397 amino acids of murine EO 
                     rotavirus VP6"
                     /protein_id="AAZ16500.1"
                     /translation="MKRGLTVAVAGAAILVAGLSGCSSNKSTDPKMDVLYSISRTLKDA
                     RDKIVEGTLYSNVSDLIQQFNQMLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLNL
                     DANYVESARTTIDYFVDFIDNVCMDEMVRESQRNGIAPQSDALRKLSGVKFRRINFNNS
                     SEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQVA
                     GFDYSCAINAPANIQQFEHIVQLRRVLTTATITLLPDAERFSFPRVINSADGATTWYFN
                     PVILRPNNVEVEFLLNGQIINTYQARFGTIVARNFDTIRLSFQLMRPPNMTPAVAALFP
                     NAQPFEHHATVGLTLRIDSAICESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWT
                     DLITNYSPSREDNLQRVFTVASIRSMLVK"
     misc_feature    4355..5548
                     /label=derived from murine EO rotavirus VP6
                     /note="derived from murine EO rotavirus VP6"
     terminator      5604..5650
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      5607..5650
                     /regulatory_class="terminator"
                     /note="rrnBT1"