LOCUS       Exported                3204 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p2059-RS-nirA, complete sequence.
ACCESSION   AY663845
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Metabolically independent and accurately adjustable Aspergillus sp. 
            expression system
  JOURNAL   Appl. Environ. Microbiol. 71 (2), 672-678 (2005)
  PUBMED    15691916
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2004) Department of Applied Plant Sciences and 
            Plant Biotechnology, BOKU - University of Natural Resources and 
            Applied Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria
REFERENCE   3  (bases 1 to 3204)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Cloning vector p2059-RS-nirA"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:285587"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(695..788)
                     /label=Aspergillus nidulans nirA promoter fragment
                     /note="Aspergillus nidulans nirA promoter fragment"
     misc_feature    complement(792..907)
                     /label=random sequence (RS) fragment derived from ampR
                     /note="random sequence (RS) fragment derived from ampR"
     misc_feature    complement(908..946)
                     /label=3xERE (estrogen responsive element)
                     /note="3xERE (estrogen responsive element)"
     primer_bind     complement(973..989)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1019..1037)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1058..1074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1082..1098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1106..1136)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1151..1172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1460..2048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2219..3079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3080..3184)
                     /gene="bla"
                     /label=AmpR promoter