LOCUS       Exported                4013 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p1cVTm, complete sequence.
ACCESSION   KY643824
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed hierarchical patterning of bacterial populations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 4013)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Cloning vector p1cVTm"
                     /mol_type="other DNA"
                     /db_xref="taxon:2049426"
     rep_origin      complement(49..637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      818..852
                     /regulatory_class="promoter"
                     /note="PJ23101*"
     regulatory      861..878
                     /regulatory_class="ribosome_binding_site"
                     /note="BBa_B0034"
     RBS             861..872
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             879..1598
                     /codon_start=1
                     /gene="mVenus"
                     /product="mVenus"
                     /label=mVenus
                     /note="yellow fluorescent protein"
                     /protein_id="ATP60647.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            879..1598
                     /gene="mVenus"
                     /label=mVenus
     regulatory      1602..1738
                     /regulatory_class="terminator"
                     /note="BBa_B0015"
     terminator      1618..1689
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1705..1732
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1748..2605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2606..2710)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2800..2843
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2846..2867
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     regulatory      complement(2868..3004)
                     /regulatory_class="terminator"
                     /note="BBa_B0015"
     terminator      2874..2901
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      2917..2988
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3008..3727)
                     /codon_start=1
                     /gene="mTurquoise2"
                     /product="mTurquoise2"
                     /label=mTurquoise2
                     /note="cyan fluorescent protein"
                     /protein_id="ATP60648.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            complement(3008..3727)
                     /gene="mTurquoise2"
                     /label=mTurquoise2
     regulatory      complement(3728..3745)
                     /regulatory_class="ribosome_binding_site"
                     /note="BBa_B0034"
     RBS             3734..3745
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      complement(3754..3788)
                     /regulatory_class="promoter"
                     /note="PJ23101"
     misc_feature    3789..3809
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3810..3867
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."