LOCUS       Exported                8290 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p119L1e, complete sequence.
ACCESSION   AY953268
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Investigation of the use of recombinant BCG, expressing the major 
            capsid protein (L1) of human papillomavirus type 16, as a candidate 
            vaccine for cervical cancer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2005) Division of Medical Virology, Department of 
            Clinical Laboratory Science and Institute of Infectious Diseases and
            Molecular Medicine, University of Cape Town, Rondebosch, Cape Town 
            7701, South Africa
REFERENCE   3  (bases 1 to 8290)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Cloning vector p119L1e"
                     /mol_type="other DNA"
                     /db_xref="taxon:319836"
     CDS             1877..2692
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6571..8181
                     /codon_start=1
                     /product="coat protein L1"
                     /label=coat protein L1
                     /note="derived from HPV-16; 3-terminal fusion with 
                     paramyxovirus SV5 antibody epitope and HIV-1 V3 BALB/c 
                     epitope"
                     /protein_id="AAX54678.1"
                     /translation="MSLWLPSEATVYLPPVPVSKVVSTDEYVARTNIYYHAGTSRLLAV
                     GHPYFPIKKPNNNKILVPKVSGLQYRVFRIHLPDPNKFGFPDTSFYNPDTQRLVWACVG
                     VEVGRGQPLGVGISGHPLLNKLDDTENASAYAANAGVDNRECISMDYKQTQLCLIGCKP
                     PIGEHWGKGSPCTNVAVNPGDCPPLELINTVIQDGDMVDTGFGAMDFTTLQANKSEVPL
                     DICTSICKYPDYIKMVSEPYGDSLFFYLRREQMFVRHLFNRAGTVGENVPDDLYIKGSG
                     STANLASSNYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRST
                     NMSLCAAISTSETTYKNTNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNSTIL
                     EDWNFGLQPPPGGTLEDTYRFVTSQAIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSAD
                     LDQFPLGRKFLLQAGLKAKPKFTLGKRKATPTTSSTSTTAKRKKRKLYRLRGPGRAFVT
                     ITFKQIPNPLLGLDRCRRS"
     terminator      8208..8254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"