LOCUS       Exported               11499 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p-4725sox10.cre, complete sequence.
ACCESSION   JQ682640
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Rodrigues FSL.M., Doughton G, Kelsh RN.
  TITLE     A novel transgenic line using the Cre-lox system to allow permanent 
            lineage labelling of the zebrafish neural crest
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11499)
  AUTHORS   Rodrigues FSL.M., Kelsh RN.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2012) Biology and Biochemistry, University of 
            Bath, Claverton Down, Bath, Somerset BA2 7AY, UK
REFERENCE   3  (bases 1 to 11499)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Cloning vector p-4725sox10.cre"
                     /mol_type="other DNA"
                     /db_xref="taxon:1177754"
     gene            27..7097
                     /gene="sox10"
                     /label=sox10
                     /note="derived from Danio rerio"
     regulatory      27..4624
                     /regulatory_class="promoter"
                     /gene="sox10"
     5UTR           join(4625..4756,6862..7097)
                     /gene="sox10"
     exon            4625..4756
                     /gene="sox10"
                     /number=1
     intron          4757..6861
                     /gene="sox10"
                     /number=1
     exon            6862..7097
                     /gene="sox10"
                     /number=2
     gene            7133..8694
                     /gene="Cre cyclization recombinase"
                     /label=Cre cyclization recombinase
     CDS             7133..8164
                     /codon_start=1
                     /gene="Cre cyclization recombinase"
                     /product="Cre cyclization recombinase"
                     /label=Cre cyclization recombinase
                     /protein_id="AFI60317.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     3UTR           8165..8694
                     /gene="Cre cyclization recombinase"
     polyA_signal    8645..8693
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(8719..8735)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8782..8800)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8821..8837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8845..8861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8869..8899)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8914..8935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9223..9811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9982..10842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10843..10947)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10973..11428)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"