LOCUS       Exported                7641 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector nvYFP w-, complete sequence.
ACCESSION   DQ835188
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Patel H, Yu MH, Sivasubramaniam G, Slovitt M, 
            Tanentzapf G, Mohanty N, Paul SM, Wu VM, Beitel GJ.
  TITLE     A new family of Drosophila balancer chromosomes with a w- dfd-GMR 
            yellow fluorescent protein marker
  JOURNAL   Genetics 174 (4), 2255-2257 (2006)
  PUBMED    17057238
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2006) Biochemistry, Molecular Biology and Cell 
            Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 
            60208, USA
REFERENCE   3  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Transformation vector nvYFP w-"
                     /mol_type="other DNA"
                     /db_xref="taxon:399388"
     regulatory      79..2813
                     /regulatory_class="promoter"
                     /note="dfd HZ2.7 promoter"
     regulatory      2819..2999
                     /regulatory_class="enhancer"
                     /note="GMR enhancer"
     CDS             3204..4052
                     /codon_start=1
                     /gene="nvYFP"
                     /allele="1"
                     /product="nuclear venus YFP"
                     /label=nvYFP
                     /note="venus (enhanced) yellow fluorescent protein with 
                     nuclear localization signal"
                     /protein_id="ABI54178.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKASKAPSRHRRHRQRSRSRNRSRSRSSERKRRQRSRSRSSERR
                     R"
     gene            3204..4052
                     /gene="nvYFP"
                     /allele="1"
                     /label=nvYFP
     CDS             3204..3920
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4196..4317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4882..5470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5641..6501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6502..6606)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7409..7641
                     /label=P element 3 end
                     /note="P element 3 end"