LOCUS       Exported               12006 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector nanos-Cre vetor, complete sequence.
ACCESSION   KC845567
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
  TITLE     Cookie monster binding to the repressed chromosomal domains is 
            needed for selective activation of testis-specific genes in 
            Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Laktionov P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Genomics lab, Institute of molecular and 
            cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 12006)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Cloning vector nanos-Cre vetor"
                     /mol_type="other DNA"
                     /db_xref="taxon:1366864"
     misc_feature    46..1022
                     /note="Drosophila melanogaster genomic DNA region upstream 
                     the nanos gene"
     CDS             1033..2064
                     /codon_start=1
                     /gene="Cre"
                     /product="Cre recombinase"
                     /label=Cre
                     /protein_id="AGR55448.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     gene            1033..2064
                     /gene="Cre"
                     /label=Cre
     misc_feature    2073..3202
                     /note="Drosophila melanogaster genomic DNA region 
                     downstream the nanos gene"
     intron          3286..3351
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3481..3501
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3773..3907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4259..7826
                     /gene="mini-white"
                     /label=mini-white
     misc_recomb     7979..8302
                     /label=phiC31 attB site
                     /note="phiC31 attB site"
     protein_bind    8005..8074
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    8428..9013
                     /label=P element 5 end
     rep_origin      complement(9248..9836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10007..10867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="AGR55449.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(10007..10867)
                     /gene="bla"
                     /label=bla
     promoter        complement(10868..10972)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    11774..12006
                     /label=P element 3 end
                     /note="P element 3 end"