| 载体基本信息 |
| 载体名称 |
mTol2-B-P-Gal4VP16-Cre-CG3 |
载体抗性 |
Ampicillin |
| 载体长度 |
9078 bp |
载体类型 |
Expression vector |
| 载体来源 |
Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, Chien CB, Dorsky R. |
mTol2-B-P-Gal4VP16-Cre-CG3 载体载体图谱
mTol2-B-P-Gal4VP16-Cre-CG3 载体载体序列
LOCUS Exported 9078 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Expression vector mTol2-B-P-Gal4VP16-Cre-CG3, complete sequence.
ACCESSION KC800700
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9078)
AUTHORS Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA,
Chien CB, Dorsky R.
TITLE Large Scale Zebrafish Gal4 Enhancer Trap Screen for Neural Specific
Expression Patterns
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9078)
AUTHORS Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA,
Chien CB, Dorsky R.
TITLE Direct Submission
JOURNAL Submitted (19-MAR-2013) Neurobiology and Anatomy, University of
Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA
REFERENCE 3 (bases 1 to 9078)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9078
/organism="Expression vector mTol2-B-P-Gal4VP16-Cre-CG3"
/mol_type="other DNA"
/db_xref="taxon:1344359"
primer_bind complement(1..18)
/label=T7 primer
/note="T7 primer"
primer_bind complement(24..41)
/label=M13F
/note="M13F"
primer_bind complement(24..40)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 112..180
/label=LacZ alpha
/note="LacZ alpha"
rep_origin 181..636
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 198..504
/label=f1(+) ori
/note="f1(+) ori"
promoter 714..818
/gene="bla"
/label=AmpR promoter
CDS 819..1679
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 1017..1679
/codon_start=1
/product="AmpR"
/label=AmpR
/protein_id="AGN90802.1"
/translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
rep_origin 1774..2456
/label=ColE1 origin
/note="ColE1 origin"
rep_origin 1850..2438
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2726..2747
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2762..2792
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_feature 2796..2818
/label=LacO
/note="LacO"
protein_bind 2800..2816
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2824..2844
/label=M13R(1)
/note="M13R(1)"
primer_bind 2824..2840
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2850..2875
/label=SP6
/note="SP6"
promoter 2858..2876
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
repeat_region 2927..3085
/label=miniTol2 3 end
/note="miniTol2 3 end"
repeat_region 2927..2943
/label=Tol2 3 TIR
/note="Tol2 3 TIR"
primer_bind 3108..3129
/label=M13R
/note="M13R"
primer_bind 3108..3124
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3144..3164
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
misc_recomb 3144..3150
/label=attR4
/note="attR4"
misc_feature 3144..3150
/label=att4_shared(1)
/note="att4_shared(1)"
misc_recomb 3151..3164
/label=attL4
/note="attL4"
misc_feature 3151..3164
/label=att4_shared
/note="att4_shared"
regulatory 3193..3203
/regulatory_class="promoter"
/note="adenovirus E1B TATA"
5UTR 3211..3242
/label=carp beta-actin trx start
/note="carp beta-actin trx start"
misc_feature 3249..3282
/label=FRT(1)
/note="FRT(1)"
protein_bind 3249..3282
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
protein_bind 3283..3307
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_recomb 3284..3290
/label=attR1
/note="attR1"
misc_feature 3288..3290
/label=att1_shared
/note="att1_shared"
misc_recomb 3291..3307
/label=attL1
/note="attL1"
misc_feature 3291..3301
/label=att1_shared(1)
/note="att1_shared(1)"
misc_feature 3313..3752
/label=Gal4 DBD
/note="Gal4 DBD"
CDS 3313..3750
/codon_start=1
/gene="Saccharomyces cerevisiae GAL4 (truncated)"
/product="DNA binding domain of the GAL4 transcriptional
activator"
/label=GAL4 DNA binding domain
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV"
misc_feature 3790..3963
/label=VP16 activation domain
/note="VP16 activation domain"
protein_bind complement(3968..3992)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_recomb 3968..3977
/label=attL2
/note="attL2"
misc_feature 3974..3977
/label=att2_shared
/note="att2_shared"
misc_recomb 3978..3991
/label=attR2
/note="attR2"
misc_feature 3978..3989
/label=att2_shared(1)
/note="att2_shared(1)"
promoter complement(3993..4010)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 4015..4212
/label=SV40 late polyA (from pCS2+)(1)
/note="SV40 late polyA (from pCS2+)(1)"
polyA_signal 4015..4149
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 4249..5597
/label=from pBS302
/note="from pBS302"
misc_feature complement(4261..4699)
/label=yeast ded1/HIS3 protein fragment X03245.1
/note="yeast ded1/HIS3 protein fragment X03245.1"
regulatory 4699..5597
/regulatory_class="other"
/note="STOP sequence"
regulatory 4700..5556
/regulatory_class="other"
/note="STOP sequence from SV40"
misc_feature 4706..5319
/label=SV40 4713..4100
/note="SV40 4713..4100"
intron 4706..4777
/note="SV40 small T antigen intron"
intron 4782..4847
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4977..4997
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 5316..5556
/label=SV40 2774..2534
/note="SV40 2774..2534"
misc_feature complement(5359..5556)
/label=SV40 late polyA (from pCS2+)(3)
/note="SV40 late polyA (from pCS2+)(3)"
polyA_signal 5422..5556
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_recomb 5598..5631
/label=FRT
/note="FRT"
misc_feature 5598..5631
/label=FRT
/note="FRT"
protein_bind 5598..5631
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
misc_feature 5645..6700
/label=iCre
/note="iCre"
CDS 5651..5671
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
protein_bind complement(6712..6732)
/gene="mutant version of attB"
/label=attB3
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
misc_recomb 6712..6718
/label=attL3
/note="attL3"
misc_feature 6712..6718
/label=att3_shared
/note="att3_shared"
misc_recomb 6719..6732
/label=attR3
/note="attR3"
misc_feature 6719..6726
/label=att3_shared(1)
/note="att3_shared(1)"
primer_bind complement(6740..6756)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6741..6756)
/label=M13F(1)
/note="M13F(1)"
misc_feature 6772..6969
/label=SV40 late polyA from pCS2+
/note="SV40 late polyA from pCS2+"
polyA_signal 6772..6906
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature complement(7028..7160)
/label=SV40 late polyA from pCS2+(2)
/note="SV40 late polyA from pCS2+(2)"
promoter 7165..7182
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(7208..7927)
/codon_start=1
/product="EGFP"
/label=EGFP
/protein_id="AGN90801.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature complement(7723..7736)
/label=EGFP-like chromophore
/note="EGFP-like chromophore"
regulatory complement(7928..8826)
/regulatory_class="promoter"
/note="cmlc2 promoter"
repeat_region 8850..9064
/label=miniTol2 5 end
/note="miniTol2 5 end"
repeat_region 9046..9064
/label=Tol2 5 TIR
/note="Tol2 5 TIR"
primer_bind complement(9074..9078)
/label=T7 primer(1)
/note="T7 primer(1)"
promoter complement(9077..17)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
