LOCUS       Exported                5527 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector LMP-1, complete sequence.
ACCESSION   HQ706129
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     A membrane leucine heptad contributes to trafficking, signaling, and
            transformation by latent membrane protein 1
  JOURNAL   J. Virol. 81 (17), 9121-9130 (2007)
  PUBMED    17581993
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2010) Department of Oncology, McArdle Laboratory 
            for Cancer Research, University of Wisconsin-Madison, 1400 
            University Ave, Madison, WI 53705, USA
REFERENCE   3  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Expression vector LMP-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:984255"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          395..967
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1022..1040
                     /regulatory_class="promoter"
                     /note="T7"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1066..2226
                     /codon_start=1
                     /product="LMP-1"
                     /label=LMP-1
                     /protein_id="ADX86837.1"
                     /translation="MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIVMS
                     DWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLMITLLLIALWNLHGQA
                     LYLGIVLFIFGCLLVLGLWIYLLEILWRLGATIWQLLAFFLAFFLDLILLIIALYLQQN
                     WWTLLVDLLWLLLFLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSNSNEG
                     RHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTDDNGPQDPDNTDDNGPHDPLPQ
                     DPDNTDDNGPQDPDNTDDNGPHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMT
                     DGGGGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYYD"
     regulatory      2118..2127
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    2523..2644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2849..3437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3608..4468)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="ADX86836.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3608..4468)
                     /gene="bla"
                     /label=bla
     promoter        complement(4469..4573)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4906..5361)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5506..5522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"