LOCUS       Exported                5395 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral cloning vector LB-G, complete sequence.
ACCESSION   EF394360
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Blo M, Bogenberger JM, Swift SE, Micklem DR, Lorens JB.
  TITLE     Expanding the spectrum of genetic elements transferable by 
            retroviral vectors
  JOURNAL   DNA Cell Biol. 26 (11), 773-779 (2007)
  PUBMED    17824835
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Lorens JB, Bogenberger JM, Swift SE, Micklem DR, Bloe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2007) Biomedicine, University of Bergen, Jonas 
            Lies vei 91, Bergen 5009, Norway
REFERENCE   3  (bases 1 to 5395)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Retroviral cloning vector LB-G"
                     /mol_type="other DNA"
                     /db_xref="taxon:421945"
     regulatory      1..24
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   25..169
                     /label=5 LTR
                     /note="5 LTR"
     misc_feature    25..93
                     /label=5 R region
                     /note="5 R region"
     misc_feature    94..169
                     /label=U5 region
                     /note="U5 region"
     regulatory      169..1079
                     /regulatory_class="other"
                     /note="MoMLV extended packaging signal (psi+)"
     misc_binding    170..187
                     /label=tRNA-Pro binding site
                     /bound_moiety="tRNA-Pro"
     misc_structure  228..252
                     /label=packaging stem-loop A
                     /note="packaging stem-loop A"
     misc_feature    232..589
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     misc_structure  302..333
                     /label=packaging stem-loop B
                     /note="packaging stem-loop B"
     misc_structure  334..376
                     /label=packaging stem-loop C
                     /note="packaging stem-loop C"
     misc_structure  379..398
                     /label=packaging stem-loop D
                     /note="packaging stem-loop D"
     CDS             645..1070
                     /codon_start=1
                     /gene="gag"
                     /product="Pr65 Gag polyprotein"
                     /label=gag
                     /note="from Moloney murine leukemia virus"
                     /protein_id="ABN09913.1"
                     /translation="MGPGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEW
                     PTFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     gene            645..1070
                     /gene="gag"
                     /label=gag
     misc_difference 651..656
                     /gene="gag"
                     /label=6bp insert compared to MoMLV
                     /note="6bp insert compared to MoMLV"
     CDS             654..1070
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1080..1449
                     /note="from MoMLV pol polyprotein; contains splice 
                     acceptor"
     regulatory      1541..1550
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1547..2266
                     /codon_start=1
                     /gene="GFP"
                     /product="enhanced green fluorescent protein"
                     /label=GFP
                     /note="EGFP"
                     /protein_id="ABN09914.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            1547..2266
                     /gene="GFP"
                     /label=GFP
     repeat_region   2323..2839
                     /label=3 LTR
                     /note="3 LTR"
     misc_feature    2323..2771
                     /label=U3 region
                     /note="U3 region"
     misc_feature    2772..2839
                     /label=3 R region
                     /note="3 R region"
     misc_feature    2839..2879
                     /label=literal polyA
                     /note="literal polyA"
     primer_bind     complement(2900..2916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2924..2940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2948..2978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2993..3014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3287..3999
                     /label=pMB1 ori (pUC)
                     /note="pMB1 ori (pUC)"
     rep_origin      complement(3302..3890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4061..4921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="ABN09912.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4061..4921)
                     /gene="bla"
                     /label=bla
     promoter        complement(4922..5026)
                     /gene="bla"
                     /label=AmpR promoter