LOCUS       Exported                4566 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector JSC, complete sequence.
ACCESSION   EU109715
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Sepulveda J, Shoemaker CB.
  TITLE     Design and testing of PCR primers for the construction of scFv 
            libraries representing the immunoglobulin repertoire of rats
  JOURNAL   J. Immunol. Methods 332 (1-2), 92-102 (2008)
  PUBMED    18242637
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Shoemaker CB, Sepulveda J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2007) Biomedical Science, Tufts Cummings School of
            Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA
REFERENCE   3  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Cloning vector JSC"
                     /mol_type="other DNA"
                     /db_xref="taxon:467236"
     regulatory      228..442
                     /regulatory_class="promoter"
                     /note="lac promoter"
     protein_bind    330..351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        366..396
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    404..420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    443..508
                     /label=pelB leader
                     /note="pelB leader"
     CDS             443..508
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     457..482
                     /label=ARAHF
                     /note="ARAHF"
     misc_feature    508..593
                     /label=multi-cloning site
                     /note="multi-cloning site"
     misc_feature    593..631
                     /label=E-tag
                     /note="E-tag"
     misc_feature    641..1855
                     /label=pIII fusion protein
                     /note="pIII fusion protein"
     primer_bind     1862..1879
                     /label=M13-UP
                     /note="M13-UP"
     primer_bind     complement(1862..1878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2091..2546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      2091..2490
                     /label=M13 origin
                     /note="M13 origin"
     promoter        2828..2932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2933..3793
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /protein_id="ABV03162.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3964..4552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3984..4566
                     /label=ColE1 origin
                     /note="ColE1 origin"