LOCUS       Exported                6460 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector JN100, complete sequence.
ACCESSION   DQ309424
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Nikodinovic J, Priestley ND.
  TITLE     A second generation snp-derived Escherichia coli-Streptomyces 
            shuttle expression vector that is generally transferable by 
            conjugation
  JOURNAL   Plasmid 56 (3), 223-227 (2006)
  PUBMED    16806469
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Nikodinovic J, Priestley ND.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2005) Chemistry, University of Montana, 32 Campus 
            Dr, Missoula, MT 59812, USA
REFERENCE   3  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Expression vector JN100"
                     /mol_type="other DNA"
                     /db_xref="taxon:360401"
     CDS             complement(191..1561)
                     /codon_start=1
                     /product="pIJ101 replication protein"
                     /label=pIJ101 replication protein
                     /protein_id="ABC02231.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDAAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     oriT            2651..2769
                     /note="from RK2 inserted in NdeI restriction site of 
                     pANT1202"
     oriT            2656..2765
                     /note="incP origin of transfer"
     primer_bind     2964..2980
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2984..3799)
                     /codon_start=1
                     /gene="aprR"
                     /product="AprR"
                     /label=aprR
                     /note="apramycin resistance protein"
                     /protein_id="ABC02230.1"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEGGMRRMRCRSPVDWLSSN"
     gene            complement(2984..3799)
                     /gene="aprR"
                     /label=aprR
     primer_bind     complement(3990..4006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4014..4030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4038..4068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4083..4104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4389..4519
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(4392..4980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5058..5215
                     /note="multiple cloning site; MCS"
     regulatory      complement(5215..5255)
                     /regulatory_class="promoter"
                     /note="snpA promoter"
     CDS             5473..6414
                     /codon_start=1
                     /gene="snpR"
                     /product="SnpR"
                     /label=snpR
                     /protein_id="ABC02232.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"
     gene            5473..6414
                     /gene="snpR"
                     /label=snpR