LOCUS       Exported                6086 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Gene silencing vector ipSilent DNA, complete sequence.
ACCESSION   AB750393
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Vu BV, Pham KT, Nakayashiki H.
  TITLE     Substrate-Induced Transcriptional Activation of the MoCel7C 
            Cellulase Gene Is Associated with Methylation of Histone H3 at 
            Lysine 4 in the Rice Blast Fungus Magnaporthe oryzae
  JOURNAL   Appl. Environ. Microbiol. 79 (21), 6823-6832 (2013)
  PUBMED    23995923
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Nakayashiki H, Vu BV.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University,
            Faculty of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, 
            Japan
REFERENCE   3  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Gene silencing vector ipSilent"
                     /mol_type="other DNA"
                     /note="ipSilent, inducible gene silencing vector for 
                     ascomycetous fungi"
                     /db_xref="taxon:1234704"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2226..2592
                     /regulatory_class="promoter"
                     /note="Aspergillus nidulans TrpC promoter"
     CDS             2593..3618
                     /codon_start=1
                     /gene="Hygr"
                     /product="hygromycin resistance gene"
                     /label=Hygr
                     /protein_id="BAM45085.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            2593..3618
                     /gene="Hygr"
                     /label=Hygr
     regulatory      3619..3864
                     /regulatory_class="terminator"
                     /note="Aspergillus nidulans TrpC terminator"
     primer_bind     3873..3889
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3892..4990
                     /regulatory_class="promoter"
                     /note="Magnaporthe oryzae MoCel7C promoter"
     misc_feature    4991..5028
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     intron          5029..5162
                     /note="Magnaporthe oryzae cutinase intron2"
     misc_feature    5163..5228
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     regulatory      5229..5432
                     /regulatory_class="terminator"
                     /note="Magnaporthe oryzae MoCel7C terminator"
     promoter        complement(5443..5461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5471..5487)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5629..6084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"