LOCUS       Exported                3007 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Transgenesis vector I-SceI-pBSII-SK+, complete sequence.
ACCESSION   DQ836146
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Thermes V, Grabher C, Ristoratore F, Bourrat F, Choulika A, 
            Wittbrodt J, Joly JS.
  TITLE     I-SceI meganuclease mediates highly efficient transgenesis in fish
  JOURNAL   Mech. Dev. 118 (1-2), 91-98 (2002)
  PUBMED    12351173
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Wittbrodt J, Rembold M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2006) Developmental Biology Unit, EMBL Heidelberg,
            Meyerhofstrasse 1, Heidelberg 69117, Germany
REFERENCE   3  (bases 1 to 3007)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Transgenesis vector I-SceI-pBSII-SK+"
                     /mol_type="other DNA"
                     /db_xref="taxon:396799"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    624..641
                     /label=I-SceI site
                     /note="I-SceI site"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    676..783
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     misc_feature    677..783
                     /note="MCS; multiple cloning site"
     primer_bind     693..709
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(743..759)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(796..814)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    821..838
                     /label=I-SceI site
                     /note="I-SceI site"
     primer_bind     complement(858..874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    882..898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(906..936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1260..1848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2019..2879)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ABH04321.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2880..2984)
                     /gene="bla"
                     /label=AmpR promoter