LOCUS       Exported                4405 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmubiSINCTIF1-pFLEV, complete sequence.
ACCESSION   KX814446
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Grant TN, De La Torre CM, Zhang N, Finer JJ.
  TITLE     Synthetic introns help identify sequences in the 5 UTR intron of 
            the Glycine max polyubiquitin (Gmubi) promoter that give increased 
            promoter activity
  JOURNAL   Planta (2017) In press
  PUBMED    28070655
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Finer J, Grant T, De La Torre C, Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4405)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Cloning vector GmubiSINCTIF1-pFLEV"
                     /mol_type="other DNA"
                     /db_xref="taxon:1955485"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      412..1151
                     /regulatory_class="promoter"
                     /note="GmubiSINCTIF1 synthetic promoter"
     CDS             1152..1871
                     /codon_start=1
                     /product="sGFP65T"
                     /label=sGFP65T
                     /protein_id="AQM73600.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      1890..2165
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      1890..2142
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2184..2200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2208..2224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2232..2262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2277..2298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2586..3174)
                     /direction=LEFT
     CDS             complement(3345..4205)
                     /codon_start=1
                     /gene="ampR"
                     /product="ampicillin resistance protein"
                     /label=ampR
                     /protein_id="AQM73599.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3345..4205)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(4206..4310)
                     /gene="bla"
                     /label=AmpR promoter