LOCUS       Exported               11450 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector Ganesh-R1, complete sequence.
ACCESSION   EF420136
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     A directional recombination cloning system for restriction- and 
            ligation-free construction of GFP, DsRed, and lacZ transgenic 
            Drosophila reporters
  JOURNAL   Gene 408 (1-2), 180-186 (2008)
  PUBMED    18077106
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Rogers CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2007) Cell & Developmental Biology, University of 
            Michigan Medical School, 3047 BSRB, 109 Zina Pitcher Place, Ann 
            Arboe, MI 48109-2200, USA
REFERENCE   3  (bases 1 to 11450)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Reporter vector Ganesh-R1"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /label=Gateway P element nuclear eGFP reporter vector
                     /note="Gateway P element nuclear eGFP reporter vector"
                     /db_xref="taxon:427136"
     misc_feature    1..233
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    1..159
                     /label=P element 3 end
                     /note="P element 3 end"
     gene            242..4247
                     /gene="white"
                     /allele="+mC"
                     /label=white
     misc_feature    4250..5964
                     /note="Gateway recombination cassette containing ccdB and 
                     CmR genes flanked by attR1/attR2 sites"
     protein_bind    4255..4379
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4404..4434
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4488..5147
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             5489..5794
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(5835..5959)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5965..6712
                     /label=spacer sequence includes partial KanR gene
                     /note="spacer sequence includes partial KanR gene"
     regulatory      6713..6846
                     /regulatory_class="promoter"
                     /note="Drosophila melanogaster Hsp70 minimal promoter"
     gene            6755..7974
                     /gene="DsRed.T4-NLS-SV40t"
                     /label=DsRed.T4-NLS-SV40t
                     /note="derived from pH-Red Stinger"
     mRNA            6755..7932
                     /gene="DsRed.T4-NLS-SV40t"
                     /product="nuclear DsRed.T4"
                     /label=DsRed.T4-NLS-SV40t mRNA
     regulatory      6914..6923
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6920..7729
                     /codon_start=1
                     /gene="DsRed.T4-NLS-SV40t"
                     /product="nuclear DsRed.T4"
                     /label=DsRed.T4-NLS-SV40t
                     /protein_id="ABN80069.1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFLYKSRHRRHRQRSRSRNRSRSRSSERKRRQRSRSRSSERRRYLYK"
     CDS             6920..7594
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     misc_feature    7598..7714
                     /gene="DsRed.T4-NLS-SV40t"
                     /label=Drosophila melanogaster tra gene NLS
                     /note="Drosophila melanogaster tra gene NLS"
     polyA_signal    7852..7973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8105..8690
                     /label=P element 5 end
                     /note="P element 5 end"
     misc_feature    8920..10949
                     /label=pUC8 plasmid sequence containing AmpR gene
                     /note="pUC8 plasmid sequence containing AmpR gene"
     rep_origin      complement(8925..9513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9684..10544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10545..10649)
                     /gene="bla"
                     /label=AmpR promoter