LOCUS       Exported                9841 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector FOXP3, complete sequence.
ACCESSION   MK012431
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Hematopoietic Stem Cell Gene Therapy for IPEX Syndrome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2018) Microbiology, Immunology and Molecular 
            Genetics, University of California, Los Angeles, 610 Charles E. 
            Young Dr. East, Los Angeles, CA 90095, USA
REFERENCE   3  (bases 1 to 9841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Expression vector FOXP3"
                     /mol_type="other DNA"
                     /db_xref="taxon:2492469"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            132..989
                     /gene="ampicillin resistance"
                     /label=ampicillin resistance
     regulatory      complement(1009..2361)
                     /regulatory_class="promoter"
                     /note="CMV"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2293..2672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2673..2876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2891..3071
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    2891..2986
                     /label=R region LTR
                     /note="R region LTR"
     misc_feature    2897..3071
                     /label=U5 region LTR
                     /note="U5 region LTR"
     misc_feature    3118..3243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3736..3969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4489..4606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      4655..4892
                     /regulatory_class="enhancer"
                     /note="CNS1"
     regulatory      4893..5252
                     /regulatory_class="enhancer"
                     /note="CNS2"
     regulatory      5252..5470
                     /regulatory_class="enhancer"
                     /note="CNS3"
     regulatory      5471..5853
                     /regulatory_class="promoter"
                     /note="FOXP3"
     5UTR           5854..6041
     gene            6042..7334
                     /gene="FOXP3"
                     /label=FOXP3
     3UTR           7338..8213
     misc_feature    8291..8331
                     /label=U3 region LTR (first half)
                     /note="U3 region LTR (first half)"
     misc_feature    8332..8597
                     /label=A2 insulator
                     /note="A2 insulator"
     misc_feature    8598..8614
                     /label=U3 region LTR (second half)
                     /note="U3 region LTR (second half)"
     LTR             8615..8795
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    8615..8710
                     /label=R region LTR
                     /note="R region LTR"
     misc_feature    8711..8794
                     /label=U5 region LTR
                     /note="U5 region LTR"
     polyA_signal    8867..8987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9027..9162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9195..9213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9223..9239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9384..9839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"