LOCUS       Exported                4131 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector CS105, complete sequence.
ACCESSION   DQ649431
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Vectors for Expression Cloning in Xenopus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 
            94720-3200, USA
REFERENCE   3  (bases 1 to 4131)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Expression vector CS105"
                     /mol_type="other DNA"
                     /db_xref="taxon:388335"
     primer_bind     34..52
                     /label=SP6
                     /note="SP6"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(150..167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    172..369
                     /label=SV40 polyA
                     /note="SV40 polyA"
     polyA_signal    172..306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(422..441)
                     /label=T3
                     /note="T3"
     promoter        complement(423..441)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(452..681,3071..3140)
                     /label=lac operon
                     /note="lac operon"
     primer_bind     complement(462..478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    486..502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(510..540)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    555..576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      808..1475
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(864..1452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1623..2483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="ABF93442.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1623..2483)
                     /gene="bla"
                     /label=bla
     promoter        complement(2484..2588)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2613..3069
                     /label=PhageM13
                     /note="PhageM13"
     rep_origin      complement(2614..3069)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     regulatory      3147..4131
                     /regulatory_class="promoter"
                     /note="CMV-IE94"
     promoter        3147..4131
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"