LOCUS       Exported                5473 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector cp9, complete sequence.
ACCESSION   AY187049
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Elias AF, Bono JL, Kupko JJ III, Stewart PE, Krum JG, Rosa P.
  TITLE     New antibiotic resistance cassettes suitable for genetic studies in 
            Borrelia burgdorferi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Krum JG, Bono JL, Elias AF, Rosa P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2002) LHBP, RML, NIAID, NIH, 903 South 4th Street,
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 5473)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Shuttle vector cp9"
                     /lab_host="Borrelia burgdorferi"
                     /mol_type="genomic DNA"
                     /note="Borrelia burgdorferi/Escherichia coli shuttle vector
                     confering gentamicin resistance"
                     /db_xref="taxon:221493"
     rep_origin      1..714
                     /label=ColE1
                     /note="ColE1"
     rep_origin      71..659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   790..973
                     /label=Borrelia burgdorferi IR2
                     /note="Borrelia burgdorferi IR2"
     CDS             complement(1178..1738)
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="Orf3; from Borrelia burgdorferi N40 plasmid cp9"
                     /protein_id="AAO62542.1"
                     /translation="MEKKRVVKVLTKKIDTYVEQNLMINESKISYYKTLKEKLNDNFKK
                     EIFHRVENIKILKEIKDNQYYKFDGYKTFLDFIKDFDVAKTQAYKYLRLATALQEGLIK
                     EDYLIENGIKNSYNFIKDKESPALKKSRQNPIKPLRFQLKTQESYDFYKKKSKFTSFMM
                     HEIFENQKDFLKKLLKKYEELKI"
     CDS             complement(1790..2341)
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="Orf2; from Borrelia burgdorferi N40 plasmid cp9"
                     /protein_id="AAO62541.1"
                     /translation="MKIGLKAARINEQKIKFPKESIFLLKEKKGDKNIYHTKILKALHR
                     VVSNKQKRCIIFFEKLLDKSEIQKLYLYPMKDGDKFLGIYYGYRKPINNVFVKYEINGV
                     ENVYTFSKIYYIEFRFKTGSVCCYLKNMRRLLRKEKIDTPYNKAIVDKFIDLEKHVYEF
                     YNKKYSSEGLILKWILKNLK"
     CDS             complement(2351..3478)
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="Orf1; from Borrelia burgdorferi N40 plasmid cp9"
                     /protein_id="AAO62540.1"
                     /translation="MENLSNINKTSKTTTCYNKLQYKLVVLISTLAYINKTYKKYTQKN
                     ILYYFNENLKRNSQTPVKLKTLQNYLYKLEKEFKVTTNYHKHLGVNLGTEIYYQLNFSK
                     KECYQKIYKYFQEKKDLRFQNRVSRGLKDRFTKNGSVDLKECLNNKNNIKEERKINEIE
                     KYQVRNYFNKCNFLCKKILSIFLTILFNLNIDKDNIIKILKIIKIIEIKLLKNKNIHFT
                     KSCMKEKQEKLKEILCNTQKEFEKNEYNSKQLETSFQKIYENYKFKPHFIIESHKYSDL
                     NNIKRKLEKSIERKKENSQQNYQDLKTNIFNILIEQLKKEVNIELLKPIIKEYLNNQKK
                     IEYNKVFCTYYCELLELMKNQKSLLSLKELNRKAI"
     repeat_region   3709..3892
                     /label=Borrelia burgdorferi IR1
                     /note="Borrelia burgdorferi IR1"
     misc_feature    4031..4491
                     /label=MCS-LacZ derived from pUC19
                     /note="MCS-LacZ derived from pUC19"
     protein_bind    4142..4163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4178..4208
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4216..4232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4240..4256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4252..4500
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRGRPR"
     misc_feature    4266..4322
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4326..4342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4498..4907
                     /regulatory_class="promoter"
                     /note="FlgB promoter"
     CDS             4908..5441
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamicin acetyltransferase-3-1"
                     /label=aacC1
                     /protein_id="AAO62543.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            4908..5441
                     /gene="aacC1"
                     /label=aacC1