LOCUS       Exported                6481 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Candida integrating vector CIp20, complete sequence.
ACCESSION   DQ015894
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Dennison PM, Ramsdale M, Manson CL, Brown AJ.
  TITLE     Gene disruption in Candida albicans using a synthetic, 
            codon-optimised Cre-loxP system
  JOURNAL   Fungal Genet. Biol. 42 (9), 737-748 (2005)
  PUBMED    16043373
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Dennison PMJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS, 
            Foresterhill, Aberdeen AB25 2ZD, UK
REFERENCE   3  (bases 1 to 6481)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Candida integrating vector CIp20"
                     /mol_type="other DNA"
                     /label=derived from Candida albicans
                     /note="derived from Candida albicans"
                     /db_xref="taxon:329970"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    670..2060
                     /label=URA3 marker
                     /note="URA3 marker"
     misc_feature    2072..3355
                     /label=HIS1 marker
                     /note="HIS1 marker"
     misc_feature    3361..4221
                     /label=RPS1
                     /note="RPS1"
     primer_bind     complement(4247..4263)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4293..4311)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4332..4348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4356..4372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4380..4410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4425..4446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4734..5322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5493..6353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6354..6458)
                     /gene="bla"
                     /label=AmpR promoter