LOCUS       Exported                5198 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Integrating vector CIp10, complete sequence.
ACCESSION   AF181970
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Murad AM, Lee PR, Broadbent ID, Barelle CJ, Brown AJ.
  TITLE     CIp10, an efficient and convenient integrating vector for Candida 
            albicans
  JOURNAL   Yeast 16 (4), 325-327 (2000)
  PUBMED    10669870
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdul Murad AM, Lee PR, Broadbent ID, Brown AJP.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-1999) Molecular and Cell Biology, University of 
            Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25
            2ZD, UK
REFERENCE   3  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Integrating vector CIp10"
                     /lab_host="Candida albicans"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:104091"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            670..2060
                     /gene="URA3"
                     /label=URA3
     primer_bind     2060..2076
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(2072..2938)
                     /gene="RP10"
                     /label=RP10
     misc_feature    2585..2590
                     /label=StuI site for targeted integration
                     /note="StuI site for targeted integration"
     misc_feature    2602..2607
                     /label=NcoI site for targeted integration
                     /note="NcoI site for targeted integration"
     misc_feature    2939..669
                     /label=pBluescript II KS+ plasmid backbone
                     /note="pBluescript II KS+ plasmid backbone"
     primer_bind     complement(2964..2980)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3010..3028)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3049..3065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3073..3089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3097..3127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3142..3163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3451..4039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4210..5070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5071..5175)
                     /gene="bla"
                     /label=AmpR promoter