LOCUS       Exported                5310 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector Aqp3a_tVBU1-Strep, complete sequence.
ACCESSION   KY379157
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Eskova A, Chauvigne F, Maischein HM, Ammelburg M, Cerda J, 
            Nusslein-Volhard C, Irion U.
  TITLE     Gain-of-function mutations of mau/DrAqp3a influence zebrafish 
            pigment pattern formation through the tissue environment
  JOURNAL   Development (2017) In press
  PUBMED    28506994
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Eskova A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2016) e-CNV group, Max Planck Institute for 
            Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 5310)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Cloning vector Aqp3a_tVBU1-Strep"
                     /mol_type="other DNA"
                     /db_xref="taxon:2005675"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      57..625
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1182..2159
                     /codon_start=1
                     /product="Aqp3aR225L-Strep fusion"
                     /label=Aqp3aR225L-Strep fusion
                     /note="tVBU1 allele"
                     /protein_id="ARU79055.1"
                     /translation="MGWQKSVLDKLAQTFQIRNKLLRQGLAECLGTLILVMFGCGSLAQ
                     LKLSEGSHGLFLTANLAFGFGATLGILVCGQVSGGHLNPAVTFALCLLGREKWRKFPVY
                     FLFQTLGSFLGAAIIFAEYHDAIYDYAGESNELLVLGEKETAGIFATYPSKYLTPLNGF
                     FDQVIGTASLIVCILAIVDPYNNPIPQGLEAFTVGFSVLIIGLSMGFNSGYAVNPARDF
                     GPLLFTAMAGWGSEVFTARDYWFLVPIFAPFIGAVIGVIVYQLMVGWHVEGEARDKKAK
                     AREEVMNLNDVASKELNSSLGSGWSHPQFEKTGHHHHHHHHGGQ"
     CDS             2094..2117
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2124..2147
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     polyA_site      2186..2241
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        3410..3514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3515..4375
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4546..5134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"