LOCUS       Exported               10139 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector Alpha-3000-gli3Act, complete sequence.
ACCESSION   KY435756
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Vega Lopez GA, Cerrizuela S, Palacio MB, Aguero TH, Tribulo C, Aybar
            MJ.
  TITLE     The gli3 transcriptional activator is a key effector of Indian 
            hedgehog signaling during Xenopus neural crest development
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Vega Lopez GA, Cerrizuela S, Palacio MB, Aguero TH, Tribulo C, Aybar
            MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2017) Developmental Biology, Instituto Superior de
            Investigaciones Biologicas (INSIBIO, CONICET-UNT), Chacabuco 461, 
            Tucuman, Tucuman T4000ILI, Argentina
REFERENCE   3  (bases 1 to 10139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: vector nti v. 11
            Sequencing Technology :: Applied Biosystems 3130 Genetic Analyzer 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Cloning vector Alpha-3000-gli3Act"
                     /mol_type="other DNA"
                     /label=sleeping beauty vector
                     /note="sleeping beauty vector"
                     /db_xref="taxon:1969589"
     regulatory      64..7119
                     /regulatory_class="promoter"
                     /gene="Xlp-Sla-3000"
     gene            64..7119
                     /gene="Xlp-Sla-3000"
                     /label=Xlp-Sla-3000
     CDS             85..3606
                     /codon_start=1
                     /gene="Xlp-Sla-3000"
                     /product="gli3-Act"
                     /label=Xlp-Sla-3000
                     /note="Activator form of GLI3 transcription factor"
                     /protein_id="ARB02201.1"
                     /translation="MPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDLLHNKRSK
                     VKPEEDHPSPGAVCIQDQPDGMTLVKEEGVKDESKQEAEVVYETNCHWEGCSREFDTQE
                     QLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCS
                     KAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPG
                     CTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQQTQGI
                     HGEHKDLSNTTSKHEECLQVRSVKTEKPMSSQPSPGGKSSCSRQQSPISNYTNSGIELN
                     LNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRVMTGTKWMEQIKVEKMKQVNGML
                     PRLNPVPPHRAPTLPPITGNGVQLNSNISLGGLAPVLPNRNDLSSMDITVLNMLNRRDS
                     NTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNLSVAGSYDPISTDASRRSSE
                     TSQCDGLPTLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDSRDCR
                     LSPLPSVNVPRRCSDGGVGSYGRRHLLPNELQVNGMRRASDPVKMVSDNVSNTRVVRFN
                     SLNNVIPPDVPPPMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLL
                     PDDMVQYLNSRNQRLYETIETDALQNNAGERNGNNFEQSHVTSNVINEQFHSSEQTDLI
                     ENKNDLPIQWNEVSSGSAEYSPSRLKYGQRFPTQQNQPFGLYNNMMVQQQNVPKSGLSQ
                     QRGYQHHTQNNPQAPQQNLDLYNNSNVWSGQLGRGNQYIDDIERSSLGHSATGSLCNSA
                     ARGQKLTTNGLPMNTGQQNFGPSTHYSVQSANRAEVVQNENIINQEFMQQMTAEHLIYG
                     MHLLGVTRSNQTTSGQNGNTTDGTRSFLSTTQNGGEQQPTLAKNFQSFVNSSGNSRQNL
                     HRNNLPYLQEQIYDTNQHIFKVNSIKMEMQSHPQQHCANAQNFSGQLYDQTATYPQQIM
                     KSGSEPGMEANCLLQESNNTNSSKLLSPGANHVTSTVDNIDNSLAGVQIAFAAIIDDGD
                     HASLISGVLSPSIIQNLSRNSSRLTTPRASLTFPAIPVSTSNMAIGDMSSLLTSLAEES
                     KFLAIMQ"
     promoter        complement(3614..3631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3636..3770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4181..4197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4205..4221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4229..4259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4274..4295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4583..5171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5342..6202)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="Ampicilin resistance"
                     /protein_id="ARB02200.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6203..6307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6781..6797
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"