LOCUS       Exported                5130 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector AF286076, complete sequence.
ACCESSION   AF286076
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Arthos J, Mullins JI.
  TITLE     Multipurpose Eukaryotic Expression Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Mullins JI, Hanley T, Kafsack BFC., Rowen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2000) Microbiology, University of Washington, HSB 
            K-455, Seattle, WA 98105, USA
REFERENCE   3  (bases 1 to 5130)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Expression vector AF286076"
                     /mol_type="other DNA"
                     /db_xref="taxon:146703"
     regulatory      16..359
                     /regulatory_class="promoter"
                     /note="SV40 early promoter/origin derived from 
                     pcDNA3.1/Zeo(+)"
     regulatory      367..2017
                     /regulatory_class="promoter"
                     /note="derived from CMV"
     regulatory      367..1001
                     /regulatory_class="enhancer"
                     /note="derived from CMV"
     enhancer        466..845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        846..1049
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1002..1006
                     /regulatory_class="CAAT_signal"
                     /note="derived from CMV"
     regulatory      1035..1040
                     /regulatory_class="TATA_box"
                     /note="derived from CMV"
     intron          1185..2008
                     /note="derived from CMV"
     misc_feature    2022..2115
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    2030..2092
                     /label=tPA leader
                     /note="tPA leader"
     misc_feature    2109..2220
                     /note="similar to SIV nef; nonfunctional"
     polyA_signal    2241..2465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      2254..2355
                     /regulatory_class="polyA_signal_sequence"
                     /note="derived from bovine growth hormone"
     misc_feature    2486..2808
                     /note="3end of neomycin resistance; nonfunctional"
     polyA_signal    2809..2943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2809..2939
                     /regulatory_class="polyA_signal_sequence"
                     /note="derived from SV40"
     primer_bind     complement(2981..2997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3001..5130
                     /label=similar to pUC19
                     /note="similar to pUC19"
     protein_bind    3005..3021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3029..3059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3074..3095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3383..3971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4142..5002)
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /protein_id="AAG45963.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_RNA        4465..5023
                     /product="ColE1 RNA II"
                     /label=replication regulation
                     /note="replication regulation"
     misc_RNA        complement(4469..4575)
                     /product="ColE1 RNA I"
                     /label=replication regulation
                     /note="replication regulation"
     promoter        complement(5003..5107)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5016..5021
                     /label=derived from ColE1
                     /note="derived from ColE1"