LOCUS       Exported                5746 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AbVec-hIgG1, complete sequence.
ACCESSION   FJ475055
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, 
            Charpilienne A, Poncet D, Wilson PC, Sollid LM.
  TITLE     Rapid Generation of Rotavirus-Specific Human Monoclonal Antibodies 
            from Small-Intestinal Mucosa
  JOURNAL   J. Immunol. 185 (9), 5377-5383 (2010)
  PUBMED    20935207
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Antibody variable gene expression vector for human IgG1 heavy chain
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2008) Medicine/Rheumatology, The University of 
            Chicago, BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 
            60637, USA
REFERENCE   4  (bases 1 to 5746)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Cloning vector AbVec-hIgG1"
                     /mol_type="other DNA"
                     /note="expression cloning vector for human antibody 
                     variable genes"
                     /db_xref="taxon:583342"
     regulatory      1..618
                     /regulatory_class="promoter"
                     /note="CMV"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    929..973
                     /note="murine IgG1 signal peptide; derived from 
                     DEC205/immunoglobluin gamma fusion protein in GenBank 
                     Accession Number DQ407610"
     misc_feature    974..991
                     /note="heavy chain variable gene cloning site; flanked by 
                     AgeI and SalI sites"
     CDS             992..1984
                     /codon_start=1
                     /gene="IgG1"
                     /product="IgG1"
                     /label=IgG1
                     /note="Cgamma-1; derived from Homo sapiens"
                     /protein_id="ACK87036.1"
                     /translation="RSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD
                     KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD
                     GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA
                     KGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL
                     DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
     gene            992..1984
                     /gene="IgG1"
                     /label=IgG1
     regulatory      1991..2000
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    2004..2138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2211..2568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2419..2554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2588..2604)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2817..3272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3604..3708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3709..4569
                     /codon_start=1
                     /gene="Amp_Res"
                     /product="beta-lactamase"
                     /label=Amp_Res
                     /note="confers resistance to ampicillin"
                     /protein_id="ACK87037.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3709..4569
                     /gene="Amp_Res"
                     /label=Amp_Res
     rep_origin      4740..5328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5652..5682
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5690..5706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5714..5730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"