LOCUS       Exported                3964 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector 35SCoreTIF1-pFLEV, complete sequence.
ACCESSION   KX814442
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Grant TN, De La Torre CM, Zhang N, Finer JJ.
  TITLE     Synthetic introns help identify sequences in the 5 UTR intron of 
            the Glycine max polyubiquitin (Gmubi) promoter that give increased 
            promoter activity
  JOURNAL   Planta (2017) In press
  PUBMED    28070655
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Finer J, Grant T, De La Torre C, Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 3964)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Cloning vector 35SCoreTIF1-pFLEV"
                     /mol_type="other DNA"
                     /db_xref="taxon:1955483"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        619..665
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     regulatory      678..687
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             684..1403
                     /codon_start=1
                     /product="sGTF65T"
                     /label=sGTF65T
                     /protein_id="AQM73592.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      1436..1711
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      1436..1688
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1743..1759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1767..1783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1791..1821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1836..1857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2145..2733)
                     /direction=LEFT
     gene            complement(2904..3764)
                     /gene="ampR"
                     /label=ampR
     CDS             complement(2904..3764)
                     /codon_start=1
                     /gene="ampR"
                     /product="ampicillin resistance protein"
                     /label=ampR
                     /protein_id="AQM73591.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3765..3869)
                     /gene="bla"
                     /label=AmpR promoter