LOCUS       Exported                4749 bp ds-DNA    circular SYN 29-11-2013
DEFINITION  Plasmid with a HIS3MX6 marker that provides a template for PCR to 
            fuse yeast enhanced GFP (yeGFP) to the C-terminus of a protein of 
            interest.
ACCESSION   .
VERSION     .
KEYWORDS    pYM44
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, 
            Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M.
  TITLE     A versatile toolbox for PCR-based tagging of yeast genes: new 
            fluorescent proteins, more markers and promoter substitution 
            cassettes.
  JOURNAL   Yeast 2004;21:947-62.
  PUBMED    15334558
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     CDS             85..798
                     /codon_start=1
                     /gene="GFP"
                     /product="yeast-enhanced green fluorescent protein"
                     /note="yeGFP"
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /db_xref="GI:1669868"
                     /protein_id="AAB18957.1"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKTDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      820..1007
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
     gene            1082..2282
                     /note="HIS3MX6"
     promoter        1082..1425
                     /note="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             1426..2079
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /function="
                     "
                     /EC_number="
                     "
                     /note="S. pombe his5"
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /experiment="
                     "
                     /protein_id="
                     "
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      2085..2282
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      complement(2663..3251)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3422..4282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4283..4387)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        4733..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"