LOCUS       Exported                4298 bp ds-DNA    circular SYN 29-11-2013
DEFINITION  Plasmid with a TRP1 marker that provides a template for PCR to fuse 
            yeast enhanced GFP (yeGFP) to the C-terminus of a protein of 
            interest.
ACCESSION   .
VERSION     .
KEYWORDS    pYM26
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, 
            Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M.
  TITLE     A versatile toolbox for PCR-based tagging of yeast genes: new 
            fluorescent proteins, more markers and promoter substitution 
            cassettes.
  JOURNAL   Yeast 2004;21:947-62.
  PUBMED    15334558
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     CDS             85..798
                     /codon_start=1
                     /gene="GFP"
                     /product="yeast-enhanced green fluorescent protein"
                     /note="yEGFP"
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /db_xref="GI:1669868"
                     /protein_id="AAB18957.1"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKTDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(886..1518)
                     /codon_start=1
                     /gene="Kluyveromyces lactis TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /note="KlTRP1"
                     /note="K. lactis auxotrophic marker that also works in S. 
                     cerevisiae"
                     /translation="MLVKVCGLQTVEAAKTAVDDGADYLGIICVPGRKRTIDSSVAKGI
                     STAVHQQENVKGTKLVGVFRNQSVDDVLQLYHEYNLDVIQLHGDEDIKEYRSLIPSSIP
                     IIKRFQFPQDCELLLDLYEHVDNVLTLFDSGEGGTGEKLNWSAISSWSASHPEIKFIIA
                     GGLNPDNVSVAINMLPNAIGVDVSGGVETDGIKDLEKVKLFIQQASQ"
     rep_origin      complement(2212..2800)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2971..3831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3832..3936)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        4282..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"