LOCUS       Exported                2512 bp ds-DNA    circular SYN 29-11-2013
DEFINITION  Precursor to plasmids carrying kanMX and other selector modules.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Wach A, Brachat A, Pohlmann R, Philippsen P.
  TITLE     New heterologous modules for classical or PCR-based gene disruptions
            in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1994;10:1793-808.
  PUBMED    7747518
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        complement(150..168)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(426..1014)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1185..2045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2046..2150)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        2496..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"