LOCUS       Exported                3503 bp ds-DNA    circular SYN 24-12-2013
DEFINITION  Plasmid for yeast gene deletion using the bleMX6 selectable marker 
            conferring phleomycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-bleMX6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM.
  TITLE     Three novel antibiotic marker cassettes for gene disruption and 
            marker switching in Schizosaccharomyces pombe.
  JOURNAL   Yeast 2005;22:1013-9.
  PUBMED    16200533
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="synthetic DNA construct"
                     /lab_host="Schizosaccharomyces pombe"
                     /mol_type="other DNA"
     gene            113..1034
                     /note="bleMX6"
                     /note="yeast selectable marker conferring phleomycin 
                     resistance"
     promoter        113..456
                     /note="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             457..831
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      837..1034
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1139..1157)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1415..2003)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2174..3034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3035..3139)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3485..3503
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"