LOCUS       Exported                4312 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Tagging vector pFA6a-5FLAG-kanMX6, complete sequence.
ACCESSION   EU493337
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E.
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe
  JOURNAL   Biotechnol J 3 (9-10), 1280-1285 (2008)
  PUBMED    18729046
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Noguchi C, Garabedian M, Malik M, Noguchi E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2008) Biochemistry and Molecular Biology, Drexel 
            University College of Medicine, 245 N 15th Street, MS 497, 
            Philadelphia, PA 19102, USA
REFERENCE   3  (bases 1 to 4312)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Tagging vector pFA6a-5FLAG-kanMX6"
                     /mol_type="other DNA"
                     /label=pFA6a derivative
                     /note="pFA6a derivative"
                     /db_xref="taxon:524039"
     CDS             66..248
                     /codon_start=1
                     /product="5 x FLAG"
                     /label=5 x FLAG
                     /protein_id="ACD03200.1"
                     /translation="MDYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK
                     HMGAPLLNKRISYDL"
     CDS             69..200
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     misc_feature    215..443
                     /label=ADH1 terminator
                     /note="ADH1 terminator"
     terminator      227..414
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            489..1845
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        489..832
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             833..1642
                     /codon_start=1
                     /gene="kanR"
                     /product="kanamycin and geneticin (G418) resistance 
                     protein"
                     /label=kanR
                     /protein_id="ACD03201.1"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            833..1642
                     /gene="kanR"
                     /label=kanR
     terminator      1648..1845
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1950..1968)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2226..2814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2985..3845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ACD03202.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2985..3845)
                     /gene="bla"
                     /label=bla
     promoter        complement(3846..3950)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4296..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"