LOCUS       Exported                3941 bp ds-DNA    circular SYN 24-12-2013
DEFINITION  Plasmid carrying the kanMX selector module conferring kanamycin 
            resistance. Also known as pFA6a-kanMX4.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6-kanMX4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Wach A, Brachat A, Pohlmann R, Philippsen P.
  TITLE     New heterologous modules for classical or PCR-based gene disruptions
            in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1994;10:1793-808.
  PUBMED    7747518
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     gene            115..1471
                     /note="kanMX"
                     /note="yeast selectable marker conferring kanamycin 
                     resistance"
     promoter        115..458
                     /note="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             459..1268
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1274..1471
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1579..1597)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1855..2443)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2614..3474)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3475..3579)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3925..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"