LOCUS       Exported                4891 bp ds-DNA    circular SYN 10-1-2013
DEFINITION  Retroviral vector for delivery and expression of libraries or 
            individual genes.
ACCESSION   .
VERSION     .
KEYWORDS    pRetro-Lib
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Clontech
  TITLE     Direct Submission
COMMENT     
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..589
                     /note="5 LTR"
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    652..851
                     /note="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /note="gag (truncated)"
                     /note="truncated MMLV gag gene lacking the start codon"
     misc_feature    1470..1502
                     /note="MCS A"
                     /note="multiple cloning site"
     misc_feature    1503..1694
                     /note="Stuffer fragment"
     misc_feature    1695..1723
                     /note="MCS B"
                     /note="multiple cloning site"
     LTR             1763..2356
                     /note="3 LTR"
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(2893..3481)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3652..4512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4513..4617)
                     /gene="bla"
                     /note="AmpR promoter"