LOCUS       Exported                5169 bp ds-DNA    circular SYN 29-11-2013
DEFINITION  Retroviral expression vector derived from the Moloney murine 
            leukemia virus. Contains a puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Morgenstern JP, Land H.
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990;18:3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Cell Biolabs
  TITLE     Direct Submission
COMMENT     Strong expression is driven by the viral promoter in the 5 LTR.
            
            The commercially provided sequence was modified to include the 
            splice donor mutation.
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     LTR             8..479
                     /note="LTR (truncated)"
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    541
                     /note="splice donor mutation"
                     /note="T-to-C mutation that blocks RNA splicing"
     misc_feature    542..741
                     /note="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /product="
                     "
                     /note="gag (truncated)"
                     /note="truncated MMLV gag gene lacking the start codon"
     misc_feature    1355..1402
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1413..1742
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1593..1728
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             1752..2351
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     LTR             2450..3043
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(3415..4003)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4181..5041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5042..5146)
                     /gene="bla"
                     /note="AmpR promoter"