LOCUS       Exported                5757 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and 
            minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent
            transcriptional activation..
ACCESSION   .
VERSION     .
KEYWORDS    pSynSRE-T-Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Dooley KA, Millinder S, Osborne TF
  TITLE     Sterol regulation of 3-hydroxy-3-methylglutaryl-coenzyme A synthase 
            gene through a direct interaction between sterol regulatory element 
            binding protein and the trimeric CCAAT-binding factor/nuclear factor
            Y.
  JOURNAL   J Biol Chem. 1998 Jan 16;273(3):1349-56.
  PUBMED    9430668
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-01-16"; volume: "273(3)"; pages: "1349-56"
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    438..572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(885..1473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(965..984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1644..2504)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2267..2286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2505..2609)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2636..3091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2723..2742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2933..2954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3281..3402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3662..5314
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3728..3746)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          5555..5620
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             join(5750..5757,1..13)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"