LOCUS       Exported               12409 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCL-MFG-BRCA1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Ruffner H, Verma IM
  TITLE     BRCA1 is a cell cycle-regulated nuclear phosphoprotein.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7138-43.
  PUBMED    9207057
REFERENCE   2  (bases 1 to 12409)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Jul 8. 94(14):7138-43."
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    823..1180
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1245..1661
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1591..1613
                     /label=pLXSN 5
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1629..1645
                     /label=pBABE 5
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1671..2045
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1983..1999
                     /label=pBMN 5
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             7889..8482
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     intron          8827..8892
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9022..9042
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9314..9448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9351..9370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9405..9424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9906..10040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9968..9987
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(10105..10122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10276..10864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10356..10375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11035..11895)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11658..11677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11896..12000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12068..12086
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     12246..12265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"