LOCUS       Exported                6323 bp ds-DNA    circular SYN 18-4-2013
DEFINITION  Superbinary intermediate vector for plant cell transformation, with 
            a spectinomycin-resistance gene.
ACCESSION   AB027256
VERSION     .
KEYWORDS    pSB11
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T.
  TITLE     Vectors carrying two separate T-DNAs for co-transformation of higher
            plants mediated by Agrobacterium tumefaciens and segregation of 
            transformants free from selection markers.
  JOURNAL   Plant J. 1996;10:165-74.
  PUBMED    8758986
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     Homologous recombination with the acceptor vector occurs in the 
            region spanning the cos and bom sites.
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     misc_feature    1..51
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    104..128
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(1304..2092)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2150..2674)
                     /codon_start=1
                     /product="streptothricin acetyltransferase"
                     /note="streptothricin acetyltransferase"
                     /protein_id="
                     "
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSPY
                     RKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSL
                     IEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMY
                     WYWFSGAQDDA"
     misc_feature    3073..3213
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3399..3987)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4495..4893
                     /note="cos"
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    6147..6171
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"