LOCUS       Exported               36909 bp ds-DNA    circular SYN 18-4-2013
DEFINITION  Superbinary acceptor vector for plant cell transformation, with a 
            tetracycline-resistance gene.
ACCESSION   AB027255
VERSION     .
KEYWORDS    pSB1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 36909)
  AUTHORS   Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T.
  TITLE     Vectors carrying two separate T-DNAs for co-transformation of higher
            plants mediated by Agrobacterium tumefaciens and segregation of 
            transformants free from selection markers.
  JOURNAL   Plant J. 1996;10:165-74.
  PUBMED    8758986
REFERENCE   2  (bases 1 to 36909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     Homologous recombination with the intermediate vector occurs in the 
            region spanning the cos and bom sites.
FEATURES             Location/Qualifiers
     source          1..36909
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     CDS             complement(353..796)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirD1"
                     /note="virD1"
                     /note="cleavage accessory protein"
                     /protein_id="
                     "
                     /translation="MSKHTRATSSETTINQHRSLKVEGFKVVSARLRSAEYETFSYQAR
                     LLGLSDSMAIRVAVRRIGGFLEIDADTREKMEAILQSIGILSSNVSMLLSAYAEDPRSD
                     LEAVRDERIAFGEAFAALDGLLRSILSVSRRRIDGRSLLKGAL"
     CDS             1065..1759
                     /gene="
                     "
                     /product="VirC1, inactivated by a frameshift mutation that 
                     eliminated the final C in codon Thr-56"
                     /note="virC1 (inactivated)"
                     /protein_id="
                     "
     CDS             1762..2370
                     /codon_start=1
                     /gene="
                     "
                     /product="VirC2"
                     /note="virC2"
                     /protein_id="
                     "
                     /translation="MAIRKPALSVGEARRLAGARPEIHHPNPTLVPQKLDLQHLPEKAD
                     EKDQQREPLVADHIYSPDRQLKLTVDALSPPPSPKKLQVFLSARPPAPQVSKTYDNLVR
                     QYSPSKSLQMILRRALDDFESMLADGSFRVAPKSYPIPSTTEKSVLVQTSRMFPVALLE
                     VARSHFDPLGLETARAFGHKLATAALASFFAGEKPSSNW"
     CDS             complement(2481..3284)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirG"
                     /note="virG"
                     /note="response regulator of virA/virG two component 
                     transduction system"
                     /protein_id="
                     "
                     /translation="MIVHPSRENFSSAVNKGSDFRLKGEPLKHVLLIDDDVAMRHLIIE
                     YLTIHAFKVTAVADSTQFTRVLSSATVDVVVVDLNLGREDGLEIVRNLAAKSDIPIIII
                     SGDRLEETDKVVALELGASDFIAKPFSTREFLARIRVALRVRPNVVRSKDRRSFCFTDW
                     TLNLRQRRLMSEAGGEVKLTAGEFNLLLAFLEKPRDVLSREQLLIASRVRDEEVYDRSI
                     DVLILRLRRKLEADPSSPQLIKTARGAGYFFDADVQVSHGGTMAA"
     CDS             complement(3416..4447)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB11"
                     /note="virB11"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MEVDPQLRFLLKPILEWLDDPKTEEIAINRPGEAFVRQAGIFTKM
                     PLPVSYDDLEDIAILAGALRKQDVGPRNPLCATELPGGERLQICLPPTVPSGTVSLTIR
                     RPSSRVSGLKEVSSRYDASRWNQWQTRRKRQNQDDEAILQHFDNGDLEAFLHACVVSRL
                     TMLLCGPTGSGKTTMSKTLISAIPPQERLITIEDTLELVIPHDNHVRLLYSKNGAGLGA
                     VSAEHLLQASLRMRPDRILLGEMRDDAAWAYLSEVVSGHPGSISTIHGANPIQGFKKLF
                     SLVKSSAQGASLEDRTLIDMLSTAIDVIIPFRAYEDVYEVGEIWLAADARRRGETIGDL
                     LNQ"
     CDS             complement(4487..5620)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB10"
                     /note="virB10"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MNNDSQQAAHEVDASGSLVSDKHRRRLSGSQKLIVGGVVLALSLS
                     LIWLGGRQKKVNENASPSTLIATNTKPFHPAPIEVPPDPPAVQEAVQPAAPLPPRGEPE
                     RHEPRPEETPIFAYSSGDQGVSKRAIQGDTGRRQEGKRDDNSLPNGEVSGENDLSIRMK
                     PTELQPSSATLLPHPDFMVTQGTIIPCILQTAIDTNLAGYVKCVLPQDIRGTTNNIVLL
                     DRGTTVVGEIQRGLQQGDGRVFVLWDRAETPDHAMISLTSPSADELGRSGLPGSVDSHF
                     WQRFSGAMLLSVVQGAFQAASTYAGSSGGGMSFNSFQNNGEQTTETALKATINIPPTLK
                     KNQGDTVSIFVARDLDFFGVYQLRLTGGATRGRNRRS"
     CDS             complement(5617..6498)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB9"
                     /note="virB9"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MIRKALFILACLFAAATGAEAEDTPMAGKLDPRMRYLAYNPDQVV
                     RLSTAVGATLVVTFATNETVTAVAVSNSKDLAALPRGNYLFFKASQVLTPQPVIVLTAS
                     DSGMRRYVFSISSKTLSHLDKEQPDLYYSVQFAYPADDAAARRREAQQKAVVDRLHAEA
                     QYQRKAENLLDQPVTALGAADSNWHYVAQGDRSLLPLEVFDNGFTTVFHFPGNVRIPSI
                     YTINPDGKEAVANYSVKGSDVEISSVSRGWRLRDGHTVLCIWNTAYDPVGQRPQTGTVR
                     PDVKRVLKGAKG"
     CDS             complement(6495..7208)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB8"
                     /note="virB8"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MTGPEYAMLVARESLAEHYKEVEAFQTARAKSARRLSKLIAAVAA
                     IAILGNVAQAFAIATMVPLSRLVPVYLWIRPDGTVDSEVSVSRLPATQEEAVVNASLWE
                     YVRLRESYDADTAQYAYDLVSNFSAPTVRQDYQQFFNYPNPSSPQVILGKRGRVEVEHI
                     ASNDVTPSTQQIRYKRTLVVDGKMPVVSTWTATVRYEKVTSLPGRLRLTNPAGLVVTSY
                     QTSEDTVSNVGHSEP"
     CDS             complement(7195..7362)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB7"
                     /note="virB7"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MKYCLLCLVVALSGCQTNDTLASCKGPIFPLNVGRWQPTPSDLQL
                     GNSGGRYDGA"
     CDS             complement(7396..8283)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB6"
                     /note="virB6"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MNFTIPAPFTAIHTIFDVAFTTGLDSMLETIQEAVSAPLIACVTL
                     WIIVQGILVIRGEVDTRSGITRVITVTIVVALIVGQANYQDYVVSIFEKTVPNFVQQFS
                     VTGLPLQTVPAQLDTMFAVTQAVFQKIASEIGPMNDQDILAFQGAQWVLYGTLWSAFGV
                     YDAVGILTKVLLAIGPLILVGYIFDRTRDIAAKWIGQLITYGLLLLLLNLVATIVILTE
                     ATALTLMLGVITFAGTTAAKIIGLYELDMFFLTGDALIVALPAIAGNIGGSYWSGATQS
                     ASSLYRRFAQVERG"
     CDS             complement(8380..9042)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB5"
                     /note="virB5"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MKTTQLIATVLTCSFLYIQPARAQFVVSDPATEAETLATALATAE
                     NLTQTIAMVTMLTSAYGVTGLLTSLNQKNQYPSTKDLDNEMFSPRMPMSTTARAITSDT
                     DRAVVGSDAEADLLRSQITGSANSAGIAADNLETMDKRLTANADTSAQLSRSRNIMQAT
                     VTNGLLLKQIHDAMIQNVQATSLLTMTTAQAGLHEAEEAAAQRKEHQKTAVIFGALP"
     CDS             complement(9057..11426)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB4"
                     /note="virB4"
                     /note="integral inner membrane ATPase"
                     /protein_id="
                     "
                     /translation="MLGASGTTERSGEIYLPYIGHLSDHIVLLEDGSIMTIARIDGVAF
                     ELEETEMRNARCRAFNTLLRNIADDHVSIYAHLVRHADVPSSAPRHFRSVFAASLNEAF
                     EQRVLSGQLLRNEHFLTLIVYPQAALGKVKRRFTKLSGKRENDLTGQIRNMEDLWHVVA
                     GSLKAYGLHRLGIREKQGVLFTEIGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGK
                     RGLEIRTPKDSYVGSIYSFREYPAKTRPGMLNALLSLDFPLVLTQSFSFLTRPQAHAKL
                     SLKSSQMLSSGDKAVTQIGKLSEAEDALASNEFVMGSHHLSLCVYADDLNSLGDRGARA
                     RTRMADAGAVVVQEGIGMEAAYWSQLPGNFKWRTRPGAITSRNFAGFVSFENFPEGASS
                     GHWGNAIARFRTNGGTPFDYIPHEHDVGMTAIFGPIGRGKTTLMMFVLAMLEQSMVDRA
                     GTVVFFDKDRGGELLVRATGGTYLALHRGTPSGLAPLRGLENTAASHDFLREWIVALIE
                     SDGRGGISPEENRRLVRGIHRQLSFDPQMRSIAGLREFLLHGPAEGAGARLQRWCRGHA
                     LGWAFDGEVDEVKLDPSITGFDMTHLLEYEEVCAPAAAYLLHRIGAMIDGRRFVMSCDE
                     FRAYLLNPKFSTVVDKFLLTVRKNNGMLILATQQPEHVLESPLGASLVAQCMTKIFYPS
                     PTADRSAYVDGLKCTEKEFQAIREDMTVGSRKFLLKRESGSVICEFDLRDMREYVAVLS
                     GRANTVRFATRLREAQEGNSSGWLSEFMARHHEAED"
     CDS             complement(11426..11752)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB3"
                     /note="virB3"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MADRLEESTLYLAATRPALFLGVPLTLAGLFMMFAGFVIVIVQNP
                     LYEVVLVPLWFAARLIVERDYNAASVVLLFLRTAGRSIDSAVWGGATVSPNPIRVPPRG
                     RGMV"
     CDS             complement(11752..12117)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB2"
                     /note="virB2"
                     /note="pilin protein precursor"
                     /protein_id="
                     "
                     /translation="MRCFERYRLHLNRLSLSNAMMRVISSCAPSLCGAIAWSISSSGPA
                     AAQSAGGGTDPATMVNNICTFILGPFGQSLAVLGIVAIGISWMFGRASLGLVAGVVGGI
                     VIMFGASFLGQTLTGGS"
     CDS             complement(12132..12851)
                     /codon_start=1
                     /gene="
                     "
                     /product="VirB1"
                     /note="virB1"
                     /note="T-DNA transfer"
                     /protein_id="
                     "
                     /translation="MLKRSGSLSLALMVSFCSSSLATPLSSAEFDHVARKCAPSVATST
                     LAAIAKVESRFDPLAIHDNTTGETLHWQDHSQATQVVRHRLDARHSLDVGLMQINSRNF
                     SMLGLTPDGALQACTSLSAAANMLKSRYAGGETIDEKQFALRRAISAYNTGNFIGGFAN
                     GYVRKVETAAQSLVPALIEPPKDDHEALKSEETWDVWGSYQRRSQEDGAGGLIAPPPPH
                     QDNGKSADDNQVLFDLY"
     misc_feature    15010..15150
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(15336..15924)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    16432..16830
                     /note="cos"
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     CDS             complement(18332..18982)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /note="TetR"
                     /protein_id="
                     "
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             19088..20287
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /note="TcR"
                     /note="confers resistance to tetracycline"
                     /protein_id="
                     "
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(21560..22708)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /note="trfA"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(26118..26489)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /note="traJ"
                     /protein_id="
                     "
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            26522..26631
                     /note="incP origin of transfer"
     operon          complement(28439..30352)
                     /operon="kfrBC"
                     /note="kfrBC operon"
     operon          complement(30253..31335)
                     /operon="kfrA"
                     /note="kfrA operon"
     operon          complement(31395..34828)
                     /operon="incC,korABFG"
                     /note="incC,korABFG operon"
     rep_origin      35754..36465
                     /note="oriV"
                     /note="incP origin of replication"